Literature summary for 2.4.1.19 extracted from

Han, R.; Ni, J.; Zhou, J.; Dong, J.; Xu, G.; Ni, Y.
Engineering of cyclodextrin glycosyltransferase reveals pH-regulated mechanism of enhanced long-chain glycosylated sophoricoside specificity (2020), Appl. Environ. Microbiol., 86, e00004-20 .

Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Paenibacillus macerans

Protein Variants

Protein Variants	Comment	Organism
A156V	the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme	Paenibacillus macerans
A156V/A166Y	the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme	Paenibacillus macerans
A156V/L174P	the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme	Paenibacillus macerans
A156V/L174P/A166Y	the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme	Paenibacillus macerans
A166Y	the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme	Paenibacillus macerans
A166Y/L174P	the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme	Paenibacillus macerans
L174P	the mutant shows increased sophoricoside glycosylation activity compared to the wild type enzyme	Paenibacillus macerans

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus macerans	P04830	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Paenibacillus macerans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
genistein + maltodextrin	less than 20% conversion ratio	Paenibacillus macerans	alpha-cyclodextrins	-	?
sophoricoside + maltodextrin	more than 40% conversion ratio	Paenibacillus macerans	Glc-sophoricoside + Glc2-sophoricoside + Glc3-sophoricoside + Glc4-sophoricoside + Glc5-sophoricoside + Glc6-sophoricoside	-	?

Subunits

Subunits	Comment	Organism
?	x * 75000, SDS-PAGE	Paenibacillus macerans

Synonyms

Synonyms	Comment	Organism
CGTase	-	Paenibacillus macerans
cyclodextrin glucosyltransferase	-	Paenibacillus macerans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.063	-	sophoricoside	wild type enzyme, at pH 6.0 and 50°C	Paenibacillus macerans
0.063	-	sophoricoside	mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C	Paenibacillus macerans
0.079	-	maltodextrin	wild type enzyme, at pH 6.0 and 50°C	Paenibacillus macerans
0.079	-	maltodextrin	mutant enzyme A156V/L174P/A166Y, at pH 6.0 and 50°C	Paenibacillus macerans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Paenibacillus macerans

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Release 2023.1 (January 2023)